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Journal of the Korean Society for Environmental Analysis 2010;13(3):143-148.
The Analysis of Residual Streptomycin in Surface Water by Liquid Chromatography-Tandem Mass Spectrometry
LC-MS/MS에 의한 하천수중 스트렙토마이신 분석
이종혁, 김병주, 명승운
경기대학교
Abstract
The method for the determination of streptomycin in environmental aqueous water samples by LC/ESI-MS/MS was developed. After ion pairing reagent was added into surface water sample, aqueous samples were extracted and concentrated through a Hydrophilic-Lipophilic Balanced (HLB) cartridge. In reverse phase liquid chromatographic (RPLC) system, chromatographic separation was achieved on a Eclipse Plus C18 column (2.1×100 mm, 5 μm), using gradient elution with 20 mM HFBA in water and acetonitrile as mobile phase. After HPLC separation, quantification and qualification were performed by electrospray ionization(ESI) and tandem mass spectrometry. As a result, calibration curve showed good linearities (above r2=0.99) in the concentration range of 0.25~1.0 μg/L. From the spiked blank surface water, the limit of detection (LOD) and the limit of quantitation (LOQ) were 0.07 μg/L and 0.23 μg/L, respectively and recovery was the range of 48.7~76%. The established method can be used to determine μg/L levels of streptomycin from the environmental aqueous sample, and also can be applied to the other environmental samples.
Key Words: streptomycin, aqueous sample, LC/ESI-MS/MS


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